杨 烨¹，于艳丽^1*，陈晓明²，陈蔚然¹，张育修¹

（1.上海杉达学院食品营养与安全系，上海201209；2.中国地质大学环境学院，武汉430074）

摘要：该研究采用多轮传代富集手段筛选青口贝中多重耐药共生菌，采用药敏纸片法分析耐药表型，利用二代测序技术进行基因组框架图测序并结合CARD数据库分析耐药基因，测定施加不同抗菌药物下菌株的生长动力曲线。结果发现，分离的Y8和Z2共生菌呈现出高度多重耐药性，分别对13种、11种抗菌药物耐药；菌株含β-内酰胺类、多肽类等耐药基因，与耐药表型基本一致；在多种药物联合施压下，生长动力受到轻微影响。为防止耐药基因的进一步传播，需加强水产品养殖中抗生素的使用管理。

关键词：耐药基因；抗生素；全基因组测序；共生菌；青口贝

中图分类号：TS254.1 文献标识码：A 文章编号：1674-506X（2024）05-0001-0009

Analysis of Antibiotic Susceptibility and Resistance Genes in Multidrug-Resistant Commensal Bacteria Isolated from Mussels

YANG Ye¹，YU Yanli^1*，CHEN Xiaoming²，CHEN Weiran¹，ZHANG Yuxiu¹

（1.Department of Food Nutrition and Safety，Sanda University，Shanghai 201209，China；2.School of Environmental Studies，China University of Geosciences，Wuhan 430074，China）

Abstract: This study employed multiple rounds of enrichment to screen for multidrug-resistant commensal bacteria in mussels. Antimicrobial resistance phenotypes were determined via the disk diffusion method, using second-generation sequencing technology for genome framework mapping sequencing and combining it with CARD database analysis of drug resistance genes. Growth kinetics curve was assessed by exposing the strains to various antimicrobial agents. The results showed that the isolated Y8 and Z2 symbiotic bacteria were highly multidrug-resistant, and they were resistant to 13 and 11 antimicrobial agents, respectively. These strains harbored resistance genes associated with β-lactams, and peptides, generally aligning with their resistance phenotypes. Furthermore, the growth kinetics of these strains displayed only limited susceptibility to the combined pressures of multiple drugs. In order to mitigate the further dissemination of resistance genes, it is necessary to enhance the management of antibiotic use in aquaculture.

Keywords：antimicrobial resistance genes；antibiotics；whole-genome sequencing；commensal bacteria；mussel

doi：10.3969/j.issn.1674-506X.2024.05-001

收稿日期：2023-10-31

基金项目：上海市“晨光计划”项目（19CGB03）；上海杉达学院科研基金项目（2024ZD02）

作者简介：杨烨（1989-），女，博士，讲师。研究方向：食品微生物学。

*通信作者：于艳丽（1982-），女，硕士，副教授。研究方向：食品质量安全检测。

引用格式：杨烨，于艳丽，陈晓明，等. 青口贝中多重耐药共生菌的药物敏感性及耐药基因分析［J］. 食品与发酵科技，

2024，60（5）：1-8，29.